Accurate, precise, rapid and economical UV Spectrophotometric first derivative method was developed for the estimation of Ampicillin Trihydrate and Cloxacillin Sodium in suspension dosage form. The principle for First - Derivative method is “the height is measured at wavelength maxima of two drugs”. Wavelengths selected for Quantitation were 216.0 nm for Ampicillin and 217.0 nm for Cloxacillin in 0.1N NaOH. Linearity observed in the concentration range was 10-30 µg/ml and 10-30 µg/ml for AMP and CLO respectively. The accuracy and precision were determined and found to comply with ICH guidelines.
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